Macedonia, which it a member of the European Union, is part of the so-called Balkans route that refugees have used on their way to rich Western countries.
The Czechs will also send financial aid of 20 million koruna ($800,000) to Macedonia.
Also Monday, the government extended a similar mission of 20 officers in Slovenia till the end of January. The Czechs had previously also deployed dozens of police officers in Hungary to help with the migrant crisis there.
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