When the boat anchored in the middle of the river for one stop, the gunmen boarded it and stole goods worth around $20,000 (£15,000).
None of the tourists, most of them American, were hurt.
The robbery happened early in the morning.
The gunmen arrived on a small boat and boarded the riverboat, threatening the crew and tourists with small calibre weapons.
The boat`s captain was hit with a pistol handle but was not seriously injured. None of the rest of the crew were hurt.
The police arrived soon after the incident and all the tourists were evacuated to a safe location.
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