The march ended on the Italian capital's Capitol Hill, where Mayor Virginia Raggi's office is located. Critics have slammed the Five Star Movement (M5S) politician over her inability to get the city's problems under control.
"Raggi, a pothole will swallow you," read one banner, as many other demonstrators called for her resignation. Six women organized the protest to air their grievances over the state of the city with 3 million residents.
The shoddiness of Rome's transit system recently made international headlines after an escalator full of football fans crashed down in an underground station, injuring dozens of people.
Raggi is currently in court over false statements she allegedly made, with a verdict expected on November 10. Should she be found guilty, the calls for her resignation will likely grow even more loud.