Ms Kyenge is an Italian citizen born in the Democratic Republic of Congo.
It is the latest in a series of rows over racism in Italy.
"I love animals - bears and wolves, as everyone knows - but when I see the pictures of Kyenge I cannot but think of, even if I`m not saying she is one, the features of an orangutan," Mr Calderoli said in a speech to a rally in the northern city of Treviso on Saturday.
He also said Ms Kyenge should be a minister "in her own country".
More about: