It follows a week which saw a bomb attack blamed on IS kill 32 people in the Turkish town of Suruc, and border clashes with the militant group.
The Iraq strikes were the first time Turkey had attacked the Kurdistan Workers` Party since a 2013 truce.
The group, also known as the PKK, has been fighting Turkey for an autonomous homeland for the Kurds for decades.
The PKK`s military wing said it killed two Turkish police officers on Wednesday. The group claims the men had collaborated with IS in the bombing in Suruc, which targeted left-wing activists.
A government statement issued on Saturday said the air force had hit PKK shelters, bunkers, storage facilities and other "logistic points" in northern Iraq, including the Qandil mountains where the PKK`s high command is based.
It did not give details of what the jets had targeted in their attacks on IS in Syria.
Turkey`s military had also shelled Islamic State and PKK positions from across the Turkish border, the statement said.
Turkey was "determined to take every step to ensure the peace and security of our people", it added.
Prime Minister Ahmet Davutoglu has said the strikes against IS are part of a broad "process".
Turkey arrested hundreds of suspected IS supporters on Friday, and has also said it will let the US use a key airbase to attack IS targets.
Fresh raids by the security forces targeting suspected IS and PKK members are reported to have taken place early on Saturday in several Turkish provinces, including Istanbul, Ankara, Konya and Manisa.
