Christian Coleman, 21, also from the U.S. took silver in a time of 9.94 seconds.
The holder of world records in three distances and an 11-time world champion, Bolt, 30, finished his final solo race in 9.95 seconds.
Twenty-seven Turkish athletes are taking part in the championships that began Friday.
Organized by the International Association of Athletics Federations (IAAF), the competition run through Aug. 13.
More about: #Ussain-bolt
