"We reject efforts to create new fait accompli in Jerusalem," Erdogan told at the 5th Summit of Heads of State of the Conference on Interaction and Confidence Building Measures in Asia (CICA) in Tajikistan's capital Dushanbe.
The conference is discussing political, economic, humanitarian and environmental issues, new challenges and threats.
Erdogan said Turkey has an active stance on Palestine issue. He also called on all the countries to respect the U.N. resolutions and the historical and legal status of Jerusalem.
Jerusalem remains at the heart of the perennial Mideast conflict, with Palestinians hoping East Jerusalem -- occupied by Israel since 1967 -- might eventually serve as the capital of an independent Palestinian state.
Erdogan also address the conflict in Syria, saying that Turkey is doing its best to end the civil war in Syria and ensure stability there. He reiterated that Turkey hosts nearly 4 million Syrians.
Erdogan said Turkey is fighting against the PKK, its Syrian affiliate People's Protection Units (YPG) and Daesh terror group, all of which threaten the future of Syria. He said efforts to eliminate one terror group by arming another, as the U.S. has done with its support for YPG in its fight against Daesh, will only result in more bloodshed.
Following his address, President Erdogan held bilateral meetings with Russian President Vladimir Putin, Iranian President Hassan Rouhani and Chiense President Xi Jinping on the sidelines of the CICA summit.
Earlier Saturday, Erdogan met with his Tajik counterpart Emomali Rahmon in Dushanbe.The meeting lasted around half an hour at the presidential palace.
Following the meeting, Erdogan and the participating heads of state and government posed for CICA family photo.